Asian Journal of Research in Chemistry

Rupali Kirtawade ¹, Pallavi Salve ¹, Chhotaram Seervi ¹, Kiran Patil ¹, Anita Kulkarni ¹, Pandurang Dhabale ¹

Affiliations
1 Govt. College of Pharmacy, Karad-415124, (Satara), Maharashtra, IN

Abstract

Two simple, accurate, precise, reproducible and economical procedures for simultaneous estimation of Atenolol (AT) and Losartan Potassium (LP) in tablet dosage form have been developed. First method based on solving of simultaneous equation using 226 nm (λmax of AT) and 208 nm (λmax of LP) as two analytical wavelengths for both drugs in Methanol solvent. Second method based on an equation of area calculation of curve at two wavelength region (231 to 221 nm and 213 to 203 nm). Linearity was observed in the concentration range of 6-36 μg/ml for AT and 2-16 μg/ml for LP. The results of analysis have been validated statistically and by recovery study.

Keywords

Atenolol, Losartan Potassium, λmax, Simultaneous Estimation Method, Area Under Curve (AUC).

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Sachin Patil ¹, B. Ramesh ¹, A. R. Hareesh ¹, Kiran Patil ², Ashok Dhokane ²

Affiliations
1 Shri Adichunchunagiri College of Pharmacy, B. G. Nagara, Tal Nagmangala, Dist Mandya, Karnataka, IN
2 Government College of Pharmacy, Vidyanagar, Karad-415124, Dist Satara, Maharashtra, IN

Abstract

Simple, precise, sensitive and reproducible quantitative reverse phase HPLC method has been developed for estimation of Sitagliptin phosphate in pharmaceutical dosage form. The method was carried out on a Phenomenex RP-18 (2) Column (250×4.6 mm i.d; particle size 5 μm) column with a mobile phase consisting of (60:40 v/v Acetonitrile:water) at a flow rate of 1.0 ml/min. The eluents were monitored at 272 nm. The retention time of Sitagliptin phosphate was found out to be 5.062. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantification. The proposed method can be used for the estimation of this drug in pharmaceutical dosage forms.

Keywords

Sitagliptin Phosphate, Validation, Estimation.

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